Janelia Fluor 549, free acid

Janelia Fluor 549，游离酸 (JF549 TFA) 是一种荧光染料，最大吸收 (Λab (max)) 为 549 nm，发射最大值 (Λem (max)) 为 571 nm。

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5mg

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6000.00

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Background

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor? HaloTag? and SNAP-Tag? ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668