2-NBDG

2NBDG

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5mg

￥938.00

450.00

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10mg

￥1580.00

850.00

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100mg

￥11250.00

7500.00

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500mg

￥25000.00

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1g

￥42000.00

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fluorescent glucose analog for visualizing glucose uptake into living cells

货号：ajci4820
CAS：186689-07-6
分子式：C12H14N4O8
分子量：342.26
纯度：＞98%

库存：现货
存储： -20°C 3 年
溶液： -80°C 6 月
-20°C 1 月

Background:
葡萄糖是一种六碳单糖，通常以游离、结合的形式，存在于动植物体内.正常人血浆中葡萄糖含量在3.89~6.11mmol/L之间，尿液中一般不含游离葡萄糖，糖尿病患者尿中的含量变化比较大。葡萄糖是维持细胞活动和组织内稳态所必需的能量来源。糖代谢是许多疾病的重要靶点，并随着病理条件的改变而改变，因此，糖代谢的评价可以作为疾病进展的重要指标。

2-NBDG是一种荧光标记的2-脱氧葡萄糖类似物，所谓的类似是指与D型葡萄糖（D-glucose）具有相同的转运体（GLUTs）和类似的胞内代谢方式，与D型葡萄糖（D-glucose）竞争性抑制，可用作细胞葡萄糖代谢的示踪剂。当它被细胞快速摄取入胞内，摄取程度与其孵育浓度在一定范围内成正相关，此过程一定时间的荧光强度反应了葡萄糖吸收的情况，以此作为探针来测定葡萄糖。

2-NBDG可用于体外多种细胞，如HepG2人肝癌细胞、L6大鼠骨骼肌细胞、MCF-7乳腺癌上皮细胞和星形胶质细胞，也可用于疾病模型、癫痫大鼠、高血糖、糖尿病或小鼠异种移植瘤模型。

2-NBDG的最大激发/发射波长为475/550nm，通过葡萄糖转运蛋白进入细胞，随后被己糖激酶磷酸化并被困在细胞内。流式细胞仪检（FL1通道）测细胞产生的荧光，检测细胞对2-NBDG的摄取，荧光微孔板法测定细胞内转运的2-NBDG浓度。它也可以用荧光成像显微镜（荧光素滤片FITC）或CCD摄像机进行检测。

恭喜以下科学家使用我司2-NBDG发表文章：

1.Hindawi.Oxidative Medicine and Cellular Longevity.Volume 2022, Article ID 9148257, 24 pages

2.J. Agric. Food Chem. 2021, 69, 13838-13848

3.Free Radical Biology and Medicine.j.freeradbiomed.2022.09.029

4.Basic & Clinical Medicine.Vol.42 No.11.November 2022

5.Cancer Cell International volume 22, Article number: 143 (2022)

6.Asian Journal of Pharmaceutical Sciences.Volume 18, Issue 5, September 2023, 100850.2-NBDG,purchased from Amgicam.DOI:10.1016/j.ajps.2023.100850

7.Biophysics Reports, 2023, 9(3): 134-145.2-NBDG,purchased from Amgicam.DOI:10.52601/bpr.2023.230003

8.Biosensors and Bioelectronics,Received 10 August 2023.2-NBDG,purchased from Wuhan Anjiekai Biological Medicine.DOI:10.1016/j.bios.2023.115820

9.Theriogenology,Volume 218, 1 April 2024, Pages 45-55.2-NBDG,purchased from Wuhan Anjiekai Biological Medicine.DOI:10.1016/j.theriogenology.2024.01.029

查看此产品更多引用产品的文献

参考文献:
[1]. Zou C, Wang Y, Shen Z. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement[J]. Journal of biochemical and biophysical methods, 2005, 64(3): 207-215.
[2]. O’Neil R G, Wu L, Mullani N. Uptake of a fluorescent deoxyglucose analog (2-NBDG) in tumor cells[J]. Molecular Imaging and Biology, 2005, 7(6): 388-392.
[3]. Tsytsarev V, Maslov K I, Yao J, et al. In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog[J]. Journal of neuroscience methods, 2012, 203(1): 136-140.
[4]. Yan Chen, Junjian Zhang, Xiang-yang Zhang, 2-NBDG as a Marker for Detecting Glucose Uptake in Reactive Astrocytes Exposed to Oxygen-Glucose Deprivation In Vitro. J Mol Neurosci (2015) 55:126–130.
[5]. Vassiliy Tsytsareva,b,1,2, Konstantin I. Maslova,1,3, Junjie Yaoa,1,3, et al, In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog, J Neurosci Methods. 2012 Jan 15;203(1):136-40.

Protocol

First, 100 μM of 2-NBDG (Amgicam, Shanghai, China) was added to the cells in each group, and then, the cells were incubated at 37 ℃ for 1h, washed with PBS three times, and finally observed under a fluorescence microscope. The mean fluorescence intensity values were analyzed using the ImageJ software.

References: 1.Free Radical Biology and Medicine.j.freeradbiomed.2022.09.029.DOI:10.1016/j.freeradbiomed.2022.09.029