TAT(YGRKKRRQRRR)是一种HIV-1病毒编码的Tat肽,可以增加异源蛋白质的产量和溶解度。
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TAT (YGRKKRRQRRR) is a HIV-1 virus-encoded Tat peptide, which can increase the yields and the solubility of heterologous proteins.
The Tat core domain contains the sequence 'YGRKKRRQRRR' and it has been demonstrated that Tat peptide fusions can increase the yields and solubility of heterologous proteins. The Tat peptide promotes the soluble expression of the membrane protein hLSECtin-CRD in E. coli without losing bioactivity and the protein expression level is not influenced compared with Tat-free proteins[1].
[1]. Dong G, et al. Tat peptide-mediated soluble expression of the membrane protein LSECtin-CRD in Escherichia coli. PLoS One. 2013 Dec 16;8(12):e83579.
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