C11 BODIPY 581/591

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C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation

货号：ajci64572
CAS：217075-36-0
分子式：C30H34BF2N2O2 ? H

分子量：504.4
纯度：＞99%
存储：Store at -20°C

溶解：30mg/ml in DMSO

库存：现货

Background:

C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation.[1] Upon oxidation, its excitation maximum shifts from 581 to 500 nm and the emission maximum shifts from 591 to 510 nm. C11 BODIPY 581/591 has been used in biochemical assays and live cell applications.[1],[2]

Reference:

[1]. Pap, E.H.W., Drummen, G.P.C., Winter, V.J., et al. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY581/591 FEBS Lett. 453(3), 278-282 (1999).
[2]. Naguib, Y.M.A. A fluorometric method for measurement of peroxyl radical scavenging activities of lipophilic antioxidants Anal. Biochem. 265(2), 290-298 (1998).

恭喜以下科学家使用我司C11 BODIPY 581/591发表SCI论文：

1.Advanced Healthcare Materials.doi:10.1002/adhm.202202150.（IF:11.0，Q1）

2.Journal of Controlled Release.doi:10.1016/j.jconrel.2023.01.035 （IF:11.467，Q1）

3.Neoplasma Vol.69, No.3, p.648–656, 2022.doi:10.4149/neo_2022_211103N1568

4.Research Square(2022).doi:10.21203/rs.3.rs-2381256/v1

5.CNS Neuroscience & Therapeutics.22 February 2023.doi:10.1111/cns.14159

6.Journal of Colloid And Interface Science.645 (2023) 882–894.doi:10.1016/j.jcis.2023.05.003

7.Nano letters.April 10, 2023.doi:10.1021/acs.nanolett.3c00365

8.Biochemistry and Biophysics Reports.Volume 36, December 2023, 101559.doi:10.1016/j.bbrep.2023.101559

9.Brain Research.Volume 1824, 1 February 2024, 148689.doi:10.1016/j.brainres.2023.148689

10.Journal of Colloid And Interface Science.20 November 2023.doi:10.1016/j.jcis.2023.11.119

11.J. Agric. Food Chem. 2023, 71, 17295?17307.doi:10.1021/acs.jafc.3c03409

12.Journal of Pharmaceutical Analysis. Available online 29 November 2023.doi:10.1016/j.jpha.2023.11.009

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Protocol

Measurement of neuron lipid peroxides:Lipid peroxides in neuron were measured by C11-BODIPY 581/591 (Amgicam, Wuhan, China; catalog number: ajci64572) staining. Briefly, cells were stained with BODIPY (5 μM) for 30 min at 37°C for 30 min in the dark. Then, cells were washed with PBS three times and observed immediately under a fluorescent microscopy. The fluorescence images were collected using a single rapid scan. In addition, we analyzed the levels of lipid peroxides by a fluorescence microplate reader. Cells were stained with BODIPY, washed with PBS and centrifugated for 5 min at 1000 ×g, the supernatant was removed, and the remaining cells were resolved with 1% Triton X-100. Fluorescence was analyzed at an excitation wavelength of 500nm and an emission wavelength of 510nm.

Reference:CNS Neuroscience & Therapeutics.22 February 2023.doi:10.1111/cns.14159