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  • 5-fluoro Orotic Acid (hydrate)
5-fluoro Orotic Acid (hydrate)的可视化放大

5-fluoro Orotic Acid (hydrate)

For selection of orotidine-5′-phosphate decarboxylase mutants of yeast

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5-fluoro Orotic Acid (hydrate)的二维码
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  • 货号: ajci69288
  • CAS: 220141-70-8
  • 别名: 5-氟乳清酸一水合物
  • 分子式: C5H3FN2O4?H2O
  • 分子量: 192.1
  • 纯度: >98%
  • 溶解度: DMF: 5 mg/ml,DMSO: 10 mg/ml,DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml
  • 储存: Store at -20°C
  • 库存: 现货

Background

5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene, which encodes orotine-5'-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.

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