Dibenzylfluorescein

A fluorogenic cytochrome P450 substrate

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库存: 现货

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数量
1mg

￥700.00

560.00

- +
5mg

￥2812.00

2250.00

- +
10mg

￥5237.00

4190.00

- +
25mg

￥11350.00

9080.00

- +

已选 0 种 0 瓶

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货号: ajci71122
CAS: 97744-44-0
别名: DBF, NSC 645658
分子式: C34H24O5
分子量: 512.6
纯度: >98%
溶解度: DMF: 25 mg/ml,DMF:PBS(pH7.2) (1:2): 0.25 mg/ml,DMSO: 10 mg/ml
储存: Store at -20°C, protect from light
库存: 现货

Background

Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). [1] [2] [3] Dibenzylfluorescein is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). [3] Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. [1] [2] [3] The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538 nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.[1] [4]?

Reference:
[1]. Stresser, D.M., Blanchard, A.P., Turner, S.D., et al. Substrate-dependent modulation of CYP3A4 catalytic activity: Analysis of 27 test compounds with four fluorometric substrates. Drug Metabolism and Disposition 28(12), 1440-1448 (2000).
[2]. Donato, M.T., Jiménez, N., Castell, J.V., et al. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab. Dispos. 32(7), 699-706 (2004).
[3]. Salminen, K.A., Lepp?nen, J., Ven?l?inen, J.I., et al. Simple, direct, and informative method for the assessment of CYP2C19 enzyme inactivation kinetics. Drug Metabolism and Disposition 39(3), 412-418 (2011).
[4]. Moutinho, D., Marohnic, C.C., Panda, S.P., et al. Altered human CYP3A4 activity caused by Antley-Bixler syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system. Drug Metabolism and Disposition 40(4), 754-760 (2012).