SBFI AM

A fluorescent Na+ indicator dye

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库存: 现货

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1mg

￥8100.00

6480.00

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货号: ajci73994
CAS: 129423-53-6
别名: Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester
分子式: C56H58N2O23
分子量: 1127.1
纯度: >98%
溶解度: Methanol: Soluble
储存: Store at -20°C
库存: 现货

Background

SBFI AM is a membrane-permeant, fluorescent Na+ indicator dye. It is selective for Na+ over K+ with Kd values of 20 and 120 mM for these ions, respectively. [1]Increasing concentration of Na+ increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm. [1]? Therefore, ratio fluorometry and imaging can be performed with the same wavelengths. SBF AM is suitable for single intracellular dye injection and is typically used for high-resolution measurements of Na+ concentration in intact tissue through two-photon confocal imaging.[2]

Reference:
[1]. Minta, A., and Tsien, R.Y. Fluorescent indicators for cytosolic sodium. The Journal of Biological Chemisty 264(32), 19449-19457 (1989).
[2]. Meier, S.D., Kovalchuk, Y., and Rose, C.R. Properties of the new fluorescent Na+ indicator CoroNa Green: Comparison with SBFI and confocal Na+ imaging. Journal of Neuroscience Methods 155(2), 251-259 (2006).