O-Propargyl-Puromycin (O-Propargylpuromycin)

A clickable form of puromycin

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库存: 现货

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500ug

￥1787.00

1430.00

- +
1mg

￥2912.00

2330.00

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Background

O-Propargyl-puromycin (OP-puro) is a clickable form of the protein synthesis inhibitor puromycin.¹ It inhibits protein synthesis in rabbit reticulocyte lysates and HEK293T cells when used at concentrations ranging from 1 to 25 μM. OP-puro is incorporated into nascent polypeptide chains where it forms covalent adducts and terminates chain elongation on the ribosome. It has been used in combination with fluorescent azides to image nascent proteins in various cells.^1,2

参考文献:
1.Liu, J., Xu, Y., Stoleru, D., et al.Imaging protein synthesis in cells and tissues with an alkyne analog of puromycinProc. Natl. Acad. Sci. USA109(2)413-418(2012)
2.Forester, C.M., Zhao, Q., Phillips, N.J., et al.Revealing nascent proteomics in signaling pathways and cell differentiationProc. Natl. Acad. Sci. USA115(10)2353-2358(2018)

Protocol

Animal experiment:

Mice: 100 μL of a 20 mM solution of O-propargyl-puromycin in PBS are injected intraperitoneally into a 3-wk-old mouse, and a mouse injected with 100 μL of PBS is used as negative control. Various organs are harvested after 1 h and are fixed in formalin overnight. Organ fragments are embedded in paraffin, sectioned, and ished with xylene to remove the paraffin. After ishing with ethanol and rehydration in TBS, the tissue sections are stained with 20 μM tetramethylrhodamine(TMR)-azide. The tissue sections are counterstained with Hoechst, mounted in standard mounting media, and are then imaged by fluorescence microscopy and DIC[1].

参考文献:

[1]. Liu J, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):413-8.