Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide)

A label for sulfhydryl-containing proteins

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10mg

￥637.00

510.00

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25mg

￥1287.00

1030.00

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50mg

￥2262.00

1810.00

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货号: ajce44288
CAS: 75350-46-8
别名: 荧光素-5-马来酰亚胺; N-(5-Fluoresceinyl)maleimide
分子式: C24H13NO7
分子量: 427.36
纯度: >98%
溶解度: DMSO : ≥ 32 mg/mL (74.88 mM)
储存: Store at -20°C, protect from light
库存: 现货

Background

Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins.^1,2,3 The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.⁴ The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.⁵

1.Curtis, S.K., and Cowden, R.R.Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedureHistochemistry68(1)23-28(1980) 2.Elliott, J.T., Tona, A., and Plant, A.L.Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopyCytometry52(2)90-100(2003) 3.Lepedda, A.j., Zinellu, A., Nieddu, G., et al.Protein sulfhydryl group oxidation and mixed-disulfide modifications in stable and unstable human carotid plaquesOxid. Med. Cell. Longev.403973(2013) 4.Bigelow, D.J., and Inesi, G.Frequency-domain fluorescence spectroscopy resolves the location of maleimide-directed spectroscopic probes within the tertiary structure of the Ca-ATPase of sarcoplasmic reticulumBiochemistry30(8)2113-2125(1991) 5.Taraska, J.W., and Zagotta, W.N.Fluorescence applications in molecular neurobiologyNeuron66(2)170-189(2010)