Sulforhodamine B acid chloride

SulforhodamineBacidchloride是形成稳定共轭物的荧光蛋白标记物.

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库存: 现货

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100mg

￥1287.00

1030.00

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货号: ajce44632
CAS: 62796-29-6
别名: 丽丝胺碱性蕊香红B磺酰氯
分子式: C27H29ClN2O6S2
分子量: 577.11
纯度: >98%
溶解度: DMSO : ≥ 100 mg/mL (173.28 mM);Water : 10 mg/mL (17.33 mM)
储存: Store at -20°C
库存: 现货

Background

Sulforhodamine B acid chloride is a fluorescent protein label forming stable conjugates.

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.