A fluorescent dye
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6-Carboxytetramethylrhodamine is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis.1,2 It displays excitation/emission maxima of 543/572 nm, respectively. 6-Carboxytetramethylrhodamine has been used in various DNA-protein binding studies, DNA FRET experiments, and as a standard reporter or quencher dye in RT-PCR.
1.Kvach, M.V., Stepanova, I.A., Prokhorenko, I.A., et al.Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probesBioconjug. Chem.20(8)1673-1682(2009) 2.Han, C., Chen, T., and Zu, L.Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridizationChem. Phys. Lett.500(4-6)323-326(2010)
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