Sulforhodamine B sodium salt (Acid Red 52)

An aminoxanthene dye

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100mg

￥537.00

430.00

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1g

￥750.00

600.00

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货号: ajce44972
CAS: 3520-42-1
别名: 磺酰罗丹明B,Acid Red 52; Kiton Red 620
分子式: C27H29N2NaO7S2
分子量: 580.65
纯度: >98%
溶解度: DMSO : 50 mg/mL (86.11 mM);Water : 20 mg/mL (34.44 mM)
储存: Store at -20°C
库存: 现货

Background

Sulforhodamine B is an aminoxanthene dye. It binds basic amino acid residues under mild acidic conditions, which can be quantified by colorimetric detection at 510 nM, and is commonly used for cell density quantification in cytotoxicity screening.^1,2

1.Skehan, P., Storeng, R., Scudiero, D., et al.New colorimetric cytotoxicity assay for anticancer-drug screeningJ. Natl. Cancer Inst.82(13)1107-1112(1990) 2.Vichai, V., and Kirtikara, K.Sulforhodamine B colorimetric assay for cytotoxicity screeningNat. Protoc.1(3)1112-1116(2006)

Protocol

Cell experiment:

Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].

参考文献:

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.