Protirelin (Synthetic thyrotropin-releasing factor)

A tropic hormone

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10mg

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货号: ajce45418
CAS: 24305-27-9
别名: 普罗瑞林; Thyrotropin-releasing-hormone; TRH
分子式: C16H22N6O4
分子量: 362.38
纯度: >98%
溶解度: DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 10 mg/ml
储存: Store at -20°C
库存: 现货

Background

Thyrotropin-releasing Hormone (TRH) is a tropic hormone that stimulates release of thyroid-stimulating hormone (TSH) and prolactin.¹ It binds to the human TRH receptor (TRHR) expressed in CHO cells and to rat TRHR in rat brain membranes (IC₅₀s = 25 and 198 nM, respectively).^2,3 TRH (1 μM) decreases polyphosphoinositide and increases arachidonic and oleic acid diacylglycerol incorporation into lipids of GH3 rat pituitary tumor cells.⁴ In vitro, it dose-dependently increases TSH release from rat anterior pituitary slices and prolactin release from cultured anterior pituitary cells.^5,6 In vivo, TRH (0.01 mg/kg) increases TSH and prolactin plasma levels in rats.¹

1.Dettmar, P.W., Lynn, A.G., Metcalf, G., et al.The ability of RX 77368 - a stabilised analogue of TRH - to provoke the secretion of prolactin and TSH in vivoNeuropeptides3(1)1-8(1982) 2.Yamada, M., Iwasaki, T., Satoh, T., et al.Activation of the thyrotropin-releasing hormone (TRH) receptor by a direct precursor of TRH, TRH-GlyNeurosci. Lett.196(1-2)109-112(1995) 3.Bhargava, H.N., and Das, S.Evidence for opiate action at the brain receptors for thyrotropin-releasing hormoneBrain Res.368(2)262-267(1986) 4.Martin, T.F.J.Thyrotropin-releasing hormone rapidly activates the phosphodiester hydrolysis of polyphosphoinositides in GH3 pituitary cells. Evidence for the role of a polyphosphoinositide-specific phospholipase C in hormone actionJ. Biol. Chem.258(24)14816-14822(1983) 5.Iriuchijima, T., Michimata, T., Miyashita, K., et al.Thyroid hormones regulate the formation of inositol phosphate in response to thyrotropin-releasing hormone in rat anterior pituitariesNeuropeptides21(1)49-53(1992) 6.Apfelbaum, M.E.Role of vasoactive intestinal peptide and 5-HT2 receptor subtype in serotonin stimulation of basal and thyrotropin-releasing-hormone-induced prolactin release in vitro from rat pituitary cellsNeuroendocrinology67(1)45-50(1998)

Protocol

Animal experiment:

Rats[2]Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

参考文献:

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074.
[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12.