Tomatidine

A steroidal alkaloid with diverse biological activities

此产品仅用于科学研究，我们不为任何个人用途提供产品和服务

库存: 现货

可选规格

包装

价格

促销价

数量
5mg

￥550.00

440.00

- +
10mg

￥762.00

610.00

- +
25mg

￥1525.00

1220.00

- +
50mg

￥2400.00

1920.00

- +
100mg

￥3775.00

3020.00

- +

已选 0 种 0 瓶

金额: ￥0.00

首页收藏

货号: ajce47536
CAS: 77-59-8
别名: 番茄碱
分子式: C27H45NO2
分子量: 415.65
纯度: >98%
溶解度: DMSO : 2.86 mg/mL (6.88 mM);Water : < 0.1 mg/mL (insoluble)
储存: Store at -20°C
库存: 现货

Background

Tomatidine is a steroidal alkaloid that has been found in the skins and leaves of tomatoes.¹ It suppresses NF-κB signaling in LPS-stimulated macrophages, blocking induced expression of inducible nitric oxide synthase (iNOS) and COX-2. Tomatidine inhibits acid sphingomyelinase activity by 84.2% when used at a concentration of 10 mM.² It also has antibacterial properties, preferentially blocking the replication of S. aureus variants that are pathogenic in cystic fibrosis over normal strains (MICs = 0.12 and >16 ?g/ml, respectively).³ It prevents skeletal muscle atrophy associated with fasting or spinal cord injury in mice.⁴ Tomatidine has also been used as a negative control for cyclopamine in studies involving signaling mediated by the hedgehog pathway.⁵

1.Chiu, F.L., and Lin, J.K.Tomatidine inhibits iNOS and COX-2 through suppression of NF-κB and JNK pathways in LPS-stimulated mouse macrophagesFEBS Lett.582(16)2407-2412(2008) 2.Kornhuber, J., Muehlbacher, M., Trapp, S., et al.Identification of novel functional inhibitors of acid sphingomyelinasePLoS One6(8)1-13(2011) 3.Mitchell, G., Gattuso, M., Grondin, G., et al.Tomatidine inhibits replication of Staphylococcus aureus small-colony variants in cystic fibrosis airway epithelial cellsAntimicrob. Agents Chemother.55(5)1937-1945(2011) 4.Dyle, M.C., Ebert, S.M., Cook, D.P., et al.Systems-based discovery of tomatidine as a natural small molecule inhibitor of skeletal muscle atrophyJ. Biol. Chem.289(21)14913-14924(2014) 5.Zhao, C., Chen, A., Jamieson, C.H., et al.Hedgehog signalling is essential for maintenance of cancer stem cells in myeloid leukaemiaNature458(7239)776-779(2009)

Protocol

Cell experiment:

RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].

参考文献:

[1]. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12.