Clofilium tosylate

Clofiliumtosylate是一种钾通道(potassiumchannel)阻滞剂，作用于人早幼粒细胞白血病(HL-60)细胞，激活caspase-3而诱导凋亡。具有抗心律失常作用。

此产品仅用于科学研究，我们不为任何个人用途提供产品和服务

库存: 现货

可选规格

包装

价格

促销价

数量
10mg

￥1200.00

960.00

- +
50mg

￥5237.00

4190.00

- +

已选 0 种 0 瓶

金额: ￥0.00

首页收藏

Background

Clofilium tosylate, a potassium channel blocker, induces apoptosis of human promyelocytic leukemia (HL-60) cells via Bcl-2-insensitive activation of caspase-3. Antiarrhythmic agent[1].

HL-60 cells treated with Clofilium (0-20 μM; 24, 48, and 72 hours) lead to suppression of viability and proliferation in both time and concentration-dependent manners. Cell viability decreases significantly in HL-60 cells treated with 2.5 μM to 10 μM of Clofilium[1].Clofilium (10 μM, 12 hours) induces the proteolytic cleavage of inactive procaspase-3, p34 into its active form, p17 and subsequent cleavage of its substrate PARP at 2 h after exposure to 10 mM Clofilium. However, there is no significant change in expression of Bcl-2 and Bax proteins[1].|| Cell Viability Assay[1]||Cell Line:|HL-60 cells |Concentration:|0-20 μM|Incubation Time:|24, 48, and 72 hours|Result:|Inhibited HL-60 cells with IC50s of 6.3 μM for 24 hours, 3.4 μM for 48 hours, 2.4 μM for 72 hours, respectively.|| Western Blot Analysis[1]||Cell Line:|HL-60 cells|Concentration:|10 μM |Incubation Time:|12 hours|Result:|Induced proteolytic cleavage of caspase-3 and subsequent cleavage of its substrate, PARP, while Bcl-2 and Bax proteins were unaltered.

[1]. Choi BY, et al. Clofilium, a potassium channel blocker, induces apoptosis of human promyelocytic leukemia (HL-60) cells via Bcl-2-insensitive activation of caspase-3. Cancer Lett. 1999 Dec 1;147(1-2):85-93.