Background
Isovitexin is a C-glycosylated flavone that has been found in Patrinia villosa and has diverse biological activities.1,2,3,4,5 It scavenges 2,2-diphenyl-1-picrylhydrazyl radicals in a cell-free assay (IC50 = 370 ?g/ml).2 Isovitexin (50 and 100 ?g/ml) is cytotoxic to HepG2 hepatic, MCF-7 breast, and HCT116 colorectal cancer cells.3 It inhibits LPS-induced production of TNF-α and IL-6 and decreases inducible nitric oxide synthase (iNOS) and COX-2 levels in RAW 264.7 cells when used at a concentration of 50 ?g/ml.4 Isovitexin (200 ?g/ml) reduces cytotoxicity induced by amyloid-β (25-35) (Aβ (25-35)) in PC12 cells.5
1.He, M., Min, J.-W., Kong, W.-L., et al.A review on the pharmacological effects of vitexin and isovitexinFitoterapia11574-85(2016)
2.Zhang, J., Yuan, K., Zhou, W.-L., et al.Studies on the active components and antioxidant activities of the extracts of Mimosa pudica Linn. from southern ChinaPharmacogn. Mag.7(25)35-39(2011)
3.Mohammed, R.S., Zeid, A.H.A., Hawary, S.S.E., et al.Flavonoid constituents, cytotoxic and antioxidant activities of Gleditsia triacanthos L. leavesSaudi J. Biol. Sci.21(6)547-553(2014)
4.Lv, H., Yu, Z., Zheng, Y., et al.Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways.Int. J. Biol. Sci.12(1)72-86(2016)
5.Guimar?es, C.C.O., D.D., Valdevite, M., Fachin Saltoratto, A.L., et al.The glycosylated flavonoids vitexin, isovitexin, and quercetrin isolated from Serjania erecta Radlk (Sapindaceae) leaves protect PC12 cells against amyloid-β25-35 peptide-induced toxicityFood Chem. Toxicol.8688-94(2015)
Protocol
Cell experiment: | Cell viability is determined by an MTT assay. RAW 264.7 cells are plated in 96-well plates (1 × 104 cells/well) and incubated with various concentrations of Isovitexin (final concentration: 0-200 μg/mL) and LPS (2 μg/mL) for 24 h. In addition, the cells are pretreated with IV (25 or 50 μg/mL) for 1 h, followed by the addition of H2O2 (300 μM). After 24 h, MTT (5 mg/mL) is added to the cells, which are then incubated for another 4 h[1]. |
Animal experiment: | Mice[1]To create an ALI model, mice are randomLy divided into six groups: control (saline), Isovitexin only (100 mg/kg, dissolved in 0.5% DMSO), LPS only (0.5 mg/kg, dissolved in saline), LPS (0.5 mg/kg) + Isovitexin (50 or 100 mg/kg), and LPS (0.5 mg/kg) + dexamethasone (Dex, 5 mg/kg dissolved in saline). Isovitexin or Dex (5 mg/kg) are administered Isovitexin. After exposure to Isovitexin or Dex for 1 h, the mice are anesthetized with diethyl ether, and LPS is administered intranasally (i.n.) to induce lung injury. After LPS administration for 12 h, the animals are euthanized. Accordingly, bronchoalveolar lavage fluid (BALF) and lung tissue samples are harvested to measure cytokine levels; ROS generation; SOD, GSH, MDA and MPO activity; and COX-2, iNOS, HO-1, and Nrf2 protein expression[1]. |
参考文献: [1]. Lv H, et al. Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MAPK and NF-κB and Activating HO-1/Nrf2 Pathways. Int J Biol Sci. 2016 Jan 1;12(1):72-86.
[2]. Hu JJ, et al. Isovitexin alleviates liver injury induced by lipopolysaccharide/d-galactosamine by activating Nrf2 and inhibiting NF-κB activation. Microb Pathog. 2018 Mar 29;119:86-92. |
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