Background
Danshensu, an active ingredient of Salvia miltiorrhiza, shows wide cardiovascular benefit by activating Nrf2 signaling pathway.
Danshensu (DSS) significantly decreases the level of the marker enzymes (creatine kinase and lactate dehydrogenase) from the coronary effluents and myocardial infarction size. This could markedly contribute to the recovery of cardiac function after I/R injury. DSS also has ROS scavenging activity and boosts endogenous antioxidants such as SOD, CAT, MDA, GSH-PX and HO-1 activities by activating nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway which is mediated by Akt and ERK1/2 in western blot analysis[2].
Acute treatment with a single dose of danshensu in rats with normal tHcy does not change plasma tHcy. In contrast, danshensu significantly lowers tHcy in rats with elevated tHcy. The relatively higher cysteine and glutathione levels after treatment with danshensu indicates that its tHcy-lowering effect is via increased activity of the trans-sulphuration pathway[1].
丹参素是丹参的一种活性成分，通过激活 Nrf2 信号通路显示出广泛的心血管益处。
丹参素 (DSS) 显着降低冠状动脉流出物中标记酶（肌酸激酶和乳酸脱氢酶）的水平和心肌梗塞面积。这可能显着有助于 I/R 损伤后心脏功能的恢复。 DSS 还具有 ROS 清除活性，并通过激活由 Akt 和 ERK1/ 2 在蛋白质印迹分析中[2]。
用单剂量丹参素对 tHcy 正常的大鼠进行急性治疗不会改变血浆 tHcy。相反，丹参素显着降低了 tHcy 升高的大鼠的 tHcy。丹参素治疗后相对较高的半胱氨酸和谷胱甘肽水平表明其降低tHcy的作用是通过增加转硫途径的活性[1]。
[1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482-490. [2]. Yu J, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 2015 Sep 15;8(9):14793-804.
Protocol
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Animal experiment:
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All chemicals are dissolved in saline, except for tolcapone which is dissolved in saline containing 20% (v/v) PEG 200. During experiments, rats are fasted overnight and randomLy assigned into different groups. About 200 μL blood is taken from orbital sinus alternatively after ethyl ether anaesthesia, and then the eye is quickly sterilized with alcohol and pressed with cotton. Blood samples are immediately collected into a polypropylene tube containing heparin-Na and centrifuged at 5000?g?at 5°C for 3 min. Prepared plasma samples are kept at ?20°C and analysed within 48 h.
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参考文献:
[1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482–490.
[2]. Yu J, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 2015 Sep 15;8(9):14793-804.
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