Tectochrysin (Techtochrysin)

A flavonoid with diverse biological activities

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5mg

￥550.00

440.00

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10mg

￥650.00

520.00

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Background

Techtochrysin is a flavonoid that has been found in T. indica and has diverse biological activities.¹,²,³ It induces adipogenesis and glucose uptake in 3T3-L1 adipocytes when used at concentrations of 12.5, 25, and 50 ?g/ml.¹ Techtochrysin is active against S. aureus, L. monocytogenes, B. subtilis, S. enterica, E. coli, V. cholera, K. pneumonia, S. dysenteriae, and P. aeruginosa (MICs = 24-64 ?g/ml).² It also scavenges peroxyl radicals in a cell-free assay when used at concentrations of 1 and 10 ?M.³

1.Hasan, M.M., Ahmed, Q.U., Soad, S.Z.M., et al.Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cellsBMC Complement. Altern. Med.17(1)431(2017) 2.Rajendran, N., Subramaniam, S., Christena, L.R., et al.Antimicrobial flavonoids isolated from Indian medicinal plant Scutellaria oblonga inhibit biofilms formed by common food pathogensNat. Prod. Res.30(17)2002-2006(2016) 3.Thao, N.P., Luyen, B.T.T., Lee, S.H., et al.Anti-osteoporotic and antioxidant activities by rhizomes of Kaempferia parviflora Wall. ex BakerNat. Prod. Sci.22(1)13-19(2016)

Protocol

Cell experiment:

Each SW480, HCT116, HT-29, A549 and MCF-7 cell line (1×104 cells) is incubated in 200 μL of RPMI 1640, DMEM medium with NSC 80687 (concentrations ranging from 1, 5, 10 μg/mL) in a 96-well flat-bottomed plate in triplicate. After incubation for 72 h at 37°C, MTT diluted in RPMI 1640, DMEM medium are added to each well and incubation is carried out for 90 min. The supernatant is then discarded and the crystal products are eluted with DMSO (200 μL/well). Colorimetric evaluation is performed with a spectrophotometer at 540 nm. The apoptosis assay is first performed by using DAPI staining. SW480 and HCT116 human colon cancer cells are cultured with concentrations of NSC 80687 (5 μg/mL), and induction of apoptotic cell death is evaluated after 24 h. Tunel assay is done[1].

Animal experiment:

Mice[1]Five-week-old male BALB/c athymic nude mice (n=10/group) are used. HCT116 cancer cells are injected subcutaneously (1×107 cells/0.1 mL PBS/animal) into the lower right flanks of mice. After 14 days, when the tumors have reached an average volume of 200-300 mm3, the tumor-bearing nude mice are intraperitoneally injected with Tectochrysin (NSC 80687) (5 mg/kg dissolved in 0.1 % DMSO) twice per week for 3 weeks. The tumor volumes are measured with vernier calipers and calculated[1].

参考文献:

[1]. Park MH, et al. Anticancer effect of Tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression. Mol Cancer. 2015 Jun 30;14:124.