Bevacizumab (Anti-Human VEGF, Humanized Antibody)

Bevacizumab (anti-VEGF, Avastin) is a humanized anti-VEGF monoclonal antibody which binds to and neutralizes all human VEGF-A isoforms and bioactive proteolytic fragments, MW:149 KD.

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1mg

￥1737.00

1390.00

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5mg

￥5937.00

4750.00

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货号: ajce52674
CAS: 216974-75-3
别名: 贝伐珠单抗 (PBS); Anti-Human VEGF, Humanized Antibody (PBS)
分子式:
分子量: 146542.45
纯度: >98%
溶解度: Soluble in DMSO
储存: Store at -80°C
库存: 现货

Background

Bevacizumab (anti-VEGF, Avastin) is a humanized anti-VEGF monoclonal antibody which binds to and neutralizes all human VEGF-A isoforms and bioactive proteolytic fragments, MW:149 KD.

Blocking VEGF with bevacizumab reduces the proangiogenic effect of affected fibroblasts in both the matrigel and direct coculture angiogenesis models.[1]

The combinational treatment of AAV9-LECT2-shRNA and bevacizumab significantly improves the therapeutic effects on liver fibrosis.[2]

[1] Hurley-Novatny AC, et al. J Invest Dermatol. 2022 Feb 19:S0022-202X(22)00125-7. [2] Lin Y, et al. Hepatology. 2021 Dec 23.

Protocol

Cell Experiment
Cell lines	NSCLC cell lines
Preparation method	Six NSCLC cell lines were seeded (~2,000–5,000 cells per well, depending on cell type) onto 96-well plates. After 24 h of incubation, cells were treated with erlotinib (0–20 μmol/L), bevacizumab (0–20 ng/mL) or a combination of these agents (erlotinib 1 μmol/L; bevacizumab 10 ng/mL) for 72 h in serum-containing medium. The viability was determined by MTS assay (Promega, Madison, WI) according to the manufacturer’s instructions.
Concentrations	0–20 ng/mL
Incubation time	72 h

Animal Experiment
Animal models	Female Wistar rats
Formulation	0.9%sodium chloride solution
Dosages	30 mg/kg
Administration	i.v.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	?Mouse??????	Rat????	Rabbit ? ?	?Guinea pig ? ? ??	?Hamster ? ?	??? Dog ? ? ?
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m²)? ? ? ? ??????????????????????	0.007	0.025	0.15	0.05	0.02	0.5
K_m factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by ??? ?Animal B Km
??????????????????????????????????????????????????????????????????????????????? Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the K_m factor for a mouse and then divide by the K_mfactor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg