G0-C14

G0-C14 是一种阳离子类脂化合物烷基修饰聚氨基胺 (PAMAM) 树状大分子。G0-C14 可用于制备一系列巨噬细胞靶向纳米颗粒 (NPs)。它可用于活性分子和疫苗递送。

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库存: 现货

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价格

促销价

数量
5mg

￥3137.00

2510.00

- +
10mg

￥4962.00

3970.00

- +
25mg

￥9950.00

7960.00

- +
50mg

￥15762.00

12610.00

- +

已选 0 种 0 瓶

金额: ￥0.00

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Background

G0-C14 is a cationic lipid-like compound alkyl-modified polyamidoamine (PAMAM) dendrimer. G0-C14 involves in the preparation of a series of macrophage-targeted nanoparticles (NPs). NPs can be used for agent and vaccine delivery^[1]^[2].

G0-C14 exhibits strong entrapment of mRNA and pDNA with an encapsulation efficiency of above 95%^[1].
Preparation of NPs^[1]:1.Dissolve PolyHCPT and DSPE-PEG3K in DMF to form a homogenous solution at a concentration of 5 mg/mL.2.Prepare a mixture of 1 nmol of siRNA (0.1 nmol/µL aqueous solution) and G0-C14 (5 mg/mL in DMF) in different N/P molar ratios. Mixed them with the polyHCPT and DSPE-PEG3K solution.3.Under vigorous stirring (1000 rpm), add the mixture dropwise to 5 mL of deionized water. 4.Transferr the formed NP dispersion to an ultrafiltration device.5.Ccentrifug at room temperature (2800 rpm×8 min) to remove the organic solvent and free compounds.

[1]. Li S, et al. Redox-responsive polyprodrug nanoparticles for targeted siRNA delivery and synergistic liver cancer therapy. Biomaterials. 2020 Mar;234:119760.
[2]. Chen Q, et al. Biodegradable nanoparticles decorated with different carbodrates for efficient macrophage-targeted gene therapy. J Control Release. 2020 Jul 10;323:179-190.